A successful cryopreservation program has become the onus of fertility clinics everywhere . Importantly, as 'freeze all' cycles are slowly making inroads, the dependence on an efficient cryopreservation program is that the need of the hour.
Vitrification has been the selection lately for cryopreservation and may have slight variations depending upon the temperature, cryo devices and therefore the brand of the media. Nevertheless, the essential principle and protocol are similar considering of these factors. Vitrification procedure is accomplished with the assistance of the available Vitrification media. The embryos are subjected to ES (Equilibration media) followed by its incorporation into VS (Vitrification media) before they're loaded on to a cryo device and plunged into nitrogen . As an embryologist, it's always been found that Vitrification is more overwhelming master than ICSI or other procedures. Unfortunately, there's not an exhaustive learning module with reference to an equivalent . Fertility clinics don't follow a typical educational program for Vitrification which can result into incompetency of the operator, poor survival rates and fluctuations in results!
Vitrification of oocytes
The final step of embryo loading is that the centerpiece of the entire procedure and therefore the entire Vitrification program rests on the premise of that crucial last one minute. Naturally, the most focus are going to be on the last step which incorporates activities like washing embryos in VS, loading them with minimal media on the cryo device and eventually draining excessive media and plunging them into nitrogen . The step isn't difficult but certainly is meticulous considering the microscopic aspects of an equivalent including the race with time.
The biggest challenge in Vitrification is that the composite of speed with time! However, the technique are often effectively implemented after an in depth stepwise thanks to learn it by self-devising an honest training environment.
Firstly, once you get familiar with the technique, just learning the protocol is not the key. There has got to be an understanding of the science behind it. it's been seen people panicking once they see embryos shrinking in ES!
Secondly, for starters, it's recommended for embryologist to be conversant in all the components of a Vitrification system. The goal here would be to try to to things perfectly without time constraints. The last major step are often broken into mini steps for better acclimatization. Embryologist can learn to regulate the coarse knob of the microscope to select up the embryos after they float due to change in viscosity in VS, the focusing of the cryotop under the microscope, the location of the hand, learning the method of washing and eventually gaining the power to load the embryos!
Thirdly, once you've got perfected the step, time will inherit the image . Then the utilization of stop watch and practicing loading in its required time becomes the main target . Initially, the loading time may exceed even two mins. With practice, slowly, you'll reduce the required time and eventually place yourself within the stipulated range of sixty to ninety seconds! This practice are often through with degenerated oocytes.
Fourthly, once you've got taken care of the time, subsequent natural factor are going to be survival! this is often the stage where you'll use discarded embryos and truly freeze them as if you're performing an actual procedure. Here, even the ES will inherit your practice and you'll observe intricate details like the reaction of the embryos with the media. At this stage, thawing technique also will be incorporated because it too contributes to the general survival. The results are often recorded and it'll give an insight into the general improvement of the operator.
Finally, once you're through with survival of embryos, it's always recommended to freeze for embryo donation patients as ED cycle harbors the simplest quality embryos. Blastocysts should be considered more within the initial phase of freezing which survives better than say day 2 or day 3 embryos. this will be furthered by increasing the amount of cryo devices progressively corresponding with checking performance of the previously frozen cryo devices simultaneously.
This comprehensive way of learning Vitrification may take six months overall depending upon the cycles happening during a fertility clinic which certainly affects the supply of gametes and resources(such as expired media for practice). It can help attain better proficiency over the technique and therefore the program are often robustly standardized during a working fertility center itself. Vitrification may be a gateway for the embryos to hibernate in an glacial period and perhaps we will make their journey qualitatively smooth!
Learn process of vitrification of embryos, Advanced course in cryopreservation, IVF training for Gynaecologist and Embryologist, Hands-on IVF IUI ICSI training courses and the way to line up IVF and IUI Laboratory in India for Gynaecologist and Embryologist at http://www.ivftraining.com
Embryology Academy for Research & Training is an IVF training institute within the field of assisted reproductive technologies which offers Short course and hands on training to young aspirants and embryologists. We at EART with our judicious mixture of varied teaching modules, lectures, actual hands-on-training and demonstrations ensure quality training much to your satisfaction. At EART our aim is to supply the foremost advanced and globally competitive training within the field of ART. Our individualized educational program , since its inception within the year 2003, has had the satisfaction of coaching 150 plus national and international candidates. EART is recognized research institute for IVF educational program for IVF / ICSI / ART Micromanipulation, Reproductive Medicine.
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